| Agency | Frequency | CH | LO | Notes | 12/21/2011 |
| Lee County SO | 37.3000 | 1 | |||
| SIRS Low band | 39.5000 | 2 | |||
| Low band Multiagency use | 39.5400 | 3 | |||
| THP Mobile Ch | 42.3600 | 4 | |||
| Tennessee Highway Patrol | 42.4200 | 5 | |||
| THP (to aid following) | 42.4200 | 6 | x | ||
| THP (to aid following) | 42.4200 | 7 | x | ||
| Sullivan County School Buses | 45.5200 | 8 | x | ||
| Tennessee Highway Patrol | 45.6200 | 9 | |||
| THP | 45.7000 | 10 | |||
| VDOT | 47.0800 | 11 | |||
| VDOT | 47.2200 | 12 | |||
| VDOT Mobile | 47.2800 | 13 | |||
| Air to Air Distress | 121.5000 | 14 | x | ||
| Wings Air to Air | 123.0250 | 15 | |||
| Air to Air Informal | 123.4500 | 16 | |||
| CAP | 150.2250 | 17 | |||
| Washington County VA FD | 151.0250 | 18 | |||
| KY GAME / FISH | 151.1450 | 19 | x | ||
| Mountain City TN PD | 151.1450 | 20 | |||
| Wise County VA VFDs | 151.2200 | 21 | |||
| NC Department of Forestry | 151.3550 | 22 | |||
| 152.8700 | 23 | x | |||
| Washington County VA SO | 153.3350 | 24 | |||
| Smyth County SO | 154.0550 | 25 | |||
| Castlewood
FD FB2 / Wautaga CO NC FDS FB2 |
154.0850 | 26 | |||
| Smyth County VA EMS | 154.1150 | 27 | |||
| Washington County FDS Glade | 154.1300 | 28 | |||
| Scott County VA VFDs | 154.3400 | 29 | |||
| Sullivan County FDs | 154.4300 | 30 | |||
| Hawkins County TN VFDs | 154.4450 | 31 | changed 11/26 | ||
| Avery Conty NC FD/EMS | 154.4450 | 32 | x | LO due to noise | |
| Virginia State Police TAC | 154.6650 | 33 | |||
| Virginia State Police Ops | 154.6950 | 34 | |||
| Scott County VA SO | 154.7400 | 35 | |||
| Greene County TN SO | 154.7850 | 36 | |||
| Ashe County NC SO | 154.8450 | 37 | x | Pager Intermod | |
| THP | 154.9050 | 38 | |||
| Unicoi County TN FD's | 154.9650 | 39 | x | Pager Intermod | |
| TEMA | 155.0250 | 40 | |||
| Russell County VA VFDs | 155.0850 | 41 | |||
| Watauga County NC SO | 155.1000 | 42 | |||
| Unicoi County TN SO | 155.1300 | 43 | |||
| Wise County EMS | 155.1750 | 44 | |||
| Virginia Mutual Aid | 155.2050 | 45 | |||
| Washington County TN Jail | 155.2500 | 46 | x | ||
| Tennessee EMS common | 155.2800 | 47 | |||
| Rogersville PD | 155.3100 | 48 | |||
| Virginia EMS Common | 155.3400 | 49 | |||
| Carilion LifeGuard Medevac | 155.3850 | 50 | |||
| Wellmont 1 Ops | 155.4000 | 51 | |||
| Washington County VA SO | 155.4900 | 52 | |||
| Carter County TN FD | 154.2950 | 53 | Previous changed due to frequent Encrypt |
||
| Russell County VA SO | 155.5500 | 54 | sometimes issues with 155.565 interf. | ||
| Wise County VA SO | 155.5650 | 55 | |||
| Johnson County TN SO | 155.5800 | 56 | |||
| Bristol VA PD | 155.5950 | 57 | Intermod Noise | ||
| Avery County NC SO | 155.6250 | 58 | |||
| Watauga Conty NC SO | 155.6550 | 59 | |||
| Hawkins County TN SO | 155.6700 | 60 | |||
| Carter County TN SO | 155.7600 | 61 | x | LO due to frequent Encrypt | |
| Tazewell County SO | 155.8050 | 62 | changed from 155.795 to avoid intermod |
||
| Hancock County EMS | 155.8950 | 63 | |||
| Smyth County VA FD's | 155.9850 | 64 | |||
| ASU Boone Campus PD | 156.0600 | 65 | |||
| Bristol VA FD’s | 156.2250 | 66 | |||
| Bristol PD Tac | 153.9650 | 67 | |||
| Virginia State Police | 159.0000 | 68 | x | ||
| TN Department Of Forestry | 159.0450 | 69 | x | Noise / LO 7/25/10 | |
| Virginia State Police | 159.1650 | 70 | |||
| NC State Parks | 159.4200 | 71 | |||
| Virginia Dept of Game / Fish | 159.4350 | 72 | x | No longer active | |
| Virginia Dept Forestry | 159.4650 | 73 | x | No longer active | |
| Sullivan Co Fire Depts | 160.1550 | 74 | |||
| Grayson County EMS/FD | 159.5350 | 75 | previous changed due to P25 | ||
| US Forestry | 169.8750 | 76 | |||
| US Forestry | 171.5750 | 77 | |||
| Air Methods Medevac | 173.3250 | 78 | |||
| US Forestry | 406.2750 | 79 | |||
| US Forestry | 406.3250 | 80 | |||
| THP | 451.5500 | 81 | |||
| Scott County VA Schools | 453.8000 | 82 | x | ||
| Wise County Schools | 460.1000 | 83 | x | ||
| TBI | 460.5250 | 84 | |||
| Medflight / Wellmont 1 Ops | 462.9500 | 85 | |||
| Bristol, VA LSC | 462.9750 | 86 | |||
| Holston Army Ammo Ops | 165.1375 | 87 | x | ||
| Holston Army Ammo Security | 165.0625 | 88 | x |